The Italian made seven changes for the Madrid derby against Atletico Madrid, which the reigning champions lost 1-0, with the match coming after their Champions League semi-final success over Manchester City.
Ancelotti will need to balance resting players and keeping them match fit over the next couple of weeks, so Thursday's team is likely to have a much more familiar look.
Eden Hazard is back into first-team training following a leg operation, but the Belgium international will again be unavailable for selection here and could ultimately miss the rest of the season.
Toni Kroos could potentially have a rest from the start, but Rodrygo might retain his spot in the final third, forming part of a front three with the returning Vinicius and Benzema, while Nacho could switch across from left-back to the middle to allow Mendy to return to the side.
Real Madrid possible starting lineup: Courtois; Vazquez, Militao, Nacho, Mendy; Valverde, Casemiro, Modric; Rodrygo, Benzema, Vinicius body check tags ::