The Uruguayan midfielder came off the bench for the final 20 minutes of El Clasico on Sunday but was withdrawn in second-half injury time after taking a blow to the knee.
As a result, Dani Ceballos and Gareth Bale are set to represent Real's only two confirmed absentees for this clash, while Luka Jovic and Isco are doubtful but would have had little chance of making the first XI anyways.
Dani Carvajal has returned from a calf problem to bolster Real's defensive ranks, but Lucas Vazquez could hold his place in an unchanged backline following his crucial strike in the dying embers of El Clasico.
With Ancelotti perhaps tempted to stick with the same XI this week given Osasuna's bright start to the season, Casemiro, Luka Modric and Toni Kroos could all reprise their roles as Eduardo Camavinga waits his turn.
Vinicius Junior and Karim Benzema could not add to their impressive opening-season tallies at Camp Nou but ought to hold their spots in the side here, while Rodrygo will hope to fend off competition from Marco Asensio for another start on the right.
Real Madrid possible starting lineup: Courtois; Vazquez, Militao, Alaba, Mendy; Kroos, Casemiro, Modric; Rodrygo, Benzema, Vinicius Jr body check tags ::