The teams have been drawn in Group B alongside Senegal and Zimbabwe, who square off in the other fixture of the opening round.
Guinea capped off their preparations for the AFCON by getting the better of Rwanda in an international friendly on Thursday.
That ended a seven-game winless run for Syli Nationale, who will be hoping to use the triumph as a turning point as they set out to enjoy a fruitful tournament in Cameroon.
First up is a crunch contest versus an opposing outfit who have won just one of the previous six meetings between the teams.
After failing to participate in the last five editions of the competition, Malawi were desperate to secure a ticket this time around and did so having picked up 10 points during the qualifiers.
The Flames have been on a woeful run of just one victory from the subsequent 11 games, which has seen them fail to qualify for November's World Cup.
Still seeking their maiden continental title, Meke Mwase's men go in search of an opening victory to set the tone for the rest of the competition.
Having never made it to the knockout stages in any of their previous appearances at this level, the East Africans will be keen to break that duck at the third time of asking.
RB Leipzig midfielder Ilaix Moriba featured in his first Guinea game last time out against Rwanda and is one to keep an eye on.
With nine goals in 17 Ligue 1 outings so far, Mohamed Bayo is expected to lead the line despite only making his debut last year.
However, Richard Mbulu, who had also tested positive for COVID-19 earlier, has now been cleared to join up with the rest of the squad.
Guinea possible starting lineup:
Keita; Sow, Camara, Conte, Kante; Keita, Diawara, Moriba; Camara, Bayo, Cisse
Malawi possible starting lineup:
Kankhobwe; Sanudi, Chirwa, Mzava, Chembezi; Idana, Chirwa, Mhone; Banda, Mbulu, Muyaba
We say: Guinea 2-1 Malawi
Guinea have more experience at this level and will be expected to come away with all three points in this one, and while we fancy Malawi to push them all the way, we are also backing Guinea to carry the day at the end of the 90 minutes.
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