Bowen's 2021-22 season saw the 25-year-old rack up 18 goals and 13 assists in 51 outings for David Moyes's side, including double figures in goals and assists in Premier League action.
Bowen was nominated for the Premier League's Player of the Season award following his stellar campaign, and the West Ham man is one of two players to have earned their maiden call-ups.
Explaining Bowen's call-up to the press on Tuesday, Gareth Southgate said: "Consistently strong performances. We were certainly thinking about involving him in March before he got injured. He is a goal threat and he is direct. His journey is really interesting in terms of the experiences he has had.
"It's a reminder you don't have to be at an elite academy and come through that pathway. Coming through the lower divisions is also a great route he worked incredibly hard for the team. He has thoroughly deserved his call-up."
Former Under-21 international Justin only made 19 appearances for Leicester throughout the campaign due to a serious knee injury, but the 24-year-old has nevertheless earned a first call-up to the senior team.
However, there is no room in the squad for Justin's Foxes teammate James Maddison, who misses out despite amassing 18 goals and 12 assists continentally and domestically throughout the campaign.
Harry Maguire earns an expected call-up despite his Manchester United struggles this term, and Marc Guehi's impressive performances for Crystal Palace have seen him earn another stint in the squad, but Eric Dier misses out.
England face a total of four Nations League games in League A Group 3 next month, firstly meeting Hungary in Budapest on June 4 before a trip to the Allianz Arena to face Germany on June 7.
Southgate's side then face Euro 2020 conquerors Italy at Molineux on June 11 before rounding off their June fixtures against Hungary in Wolverhampton three days later.
Goalkeepers: Jordan Pickford (Everton), Nick Pope (Burnley), Aaron Ramsdale (Arsenal)
Defenders: Trent Alexander-Arnold (Liverpool), Conor Coady (Wolverhampton Wanderers), Marc Guehi (Crystal Palace), Reece James (Chelsea), James Justin (Leicester City) Harry Maguire (Manchester United), John Stones (Manchester City), Fikayo Tomori (AC Milan), Kieran Trippier (Newcastle United), Kyle Walker (Manchester City), Ben White (Arsenal)
Midfielders: Jude Bellingham (Borussia Dortmund), Conor Gallagher (Crystal Palace, loan from Chelsea), Mason Mount (Chelsea), Kalvin Phillips (Leeds United), Declan Rice (West Ham United), James Ward-Prowse (Southampton)
Forwards: Tammy Abraham (AS Roma), Jarrod Bowen (West Ham United), Phil Foden (Manchester City), Jack Grealish (Manchester City), Harry Kane (Tottenham Hotspur), Bukayo Saka (Arsenal), Raheem Sterling (Manchester City) body check tags ::